SEMINAR ON BACTERIOLOGICAL ANALYSIS OF ZOBO DRINK SOLD IN OWERRI AND AROUND FEDERAL POLYTECHNIC NEKEDE AND WITHIN OWERRI METROPOLIS

BACTERIOLOGICAL ANALYSIS OF ZOBO DRINK SOLD IN OWERRI AND AROUND FEDERAL POLYTECHNIC NEKEDE AND WITHIN OWERRI METROPOLIS BY NLEMCHUKWU IFEOMA PRISCA 12/ 0027/ ST OBAJI EMMANUELLA U. 12/ 0028/ ST EZEAKUNNE KELECHI VIRGINIA 12/ 0029/ ST AGWULONU PRISCA CHIDIMMA 12/ 0030/ ST A PROJECT WORK SUBMITTED TO THE DEPARTMENT OF SCIENCE LABORATORY TECHNOLOGY, SCHOOL OF INDUSTRIAL AND APPLIED SCIENCES, FEDERAL POLYTECHNIC NEKEDE, OWERRI - IMO STATE. IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF NATIONAL DIPLOMA (ND) IN SCIENCE LABORATORY TECHNOLOGY DECEMBER, 2014. CERTIFICATION This project had been accepted and approved by the Department of Science Laboratory Technology of the School of Industrial and Applied Sciences of Federal Polytechnic Nekede, Owerri in partial fulfillment of the requirements, for Award of the National Diploma Certificate in Science Laboratory Technology. ……………………………… ………………………… Dr. (Mrs.) Ibe I.J. Date (Project Supervisor) ……………………………… ………………………… Mr. Nwanze P.I. Date (Head of Department) ……………………………… ………………………… External Examiner Date DEDICATION This project is dedicated to the Most High God for His unspeakable grace, mercy, guidance, protection and divine supply throughout the course of this work. We also dedicate this project to all Nigerian Students.   ACKNOWLEDGEMENT We are grateful to the Most High God for His unimaginative assistance throughout our stay in this school. Our profound thanks go to our parents, sponsors, guardians for their support and assistance both financially and otherwise. This work will be incomplete if we fail to acknowledge our supervisor, Dr. (Mrs.) Ibe I.J, whose ideas and suggestions made this work a success. We extend our gratitude to our beloved siblings, friends, course-mates, well-wishers and to all Nigerian students for their prayers, care, love, encouragement and assistance throughout the course of this work. We also appreciate the authors of various books whose write-ups contributed immensely to the success of this project. God bless you all. ABSTRACT The Bacteriological Quality Of ‘Zobo’ Drink Sold In Owerri Metropolis was analyzed using standard microbiological methods. The organisms isolated were Staphylococcus aureus, Streptococcus species and Escherichia coli. The total viable count of the samples ranged from 0.3 x 109cfu/ml to 4.1 x 1011cfu/ml, while the samples bought from Nekede market had colonies that were too numerous to count. These micro-organisms isolated could be as a result of poor water quality, unhygienic handling and packaging. Care should be taken to address these factors to promote public health and reduce cases of food poisoning.   TABLE OF CONTENTS The Page - - - - - - - - - i Certification- - - - - - - - - ii Dedication - - - - - - - - - iii Acknowledgement - - - - - - - iv Abstract - - - - - - - - - v Table of Contents - - - - - - - vi CHAPTER ONE 1.0 Introduction - - - - - - - 1 Chapter Two 2.0 Literature Review - - - - - - - 4 Chapter Three 3.0 Materials and Methods - - - - - - 9 3.1 Sample Collection - - - - - - - 9 3.2 Sample Processing and Culture - - - - 9 3.3 Identification of Bacterial Isolates - - - - 10 Chapter Four 4.0 Results and Discussion 4.1 Results - - - - - - - - 12 4.2 Discussions - - - - - - - 15 Chapter Five 5.0 Conclusion and Recommendations 5.1 Conclusion- - - - - - - - 17 5.1 Recommendations - - - - - - 17 References - - - - - - - 18   FOR FULL MATERIAL CALL 08032849308 CHINEDU